Dr. Vasa Radonic
Project coordinator
Location:
BioSense Institute, University of Novi Sad
Dr. Zorana Đinđića 1, 21000 Novi Sad, Serbia
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Presenters
Dr. Aphrodite Kalogianni
Post-doc Researcher
Agricultural University of Athens, Greece
Dr. Aphrodite Kalogianni has graduated from the School of Veterinary Medicine at the Aristotle University of Thessaloniki. She holds a Master’s Degree in Quality Management and Technology from the Hellenic Open University and completed her doctoral thesis in the Laboratory of Anatomy and Physiology of Farm Animals at the Department of Animal Science, Agricultural University of Athens (AUA). Her PhD research focused on the epidemiological investigation, diagnostic approaches, risk factor assessment, and impacts of small ruminant lentiviruses in intensively reared dairy sheep. She has participated in numerous research projects co-funded by the European Commission and the Hellenic Foundation for Research and Innovation and she has 13 publications in scientific journals and 28 presentations in peer-reviewed international conference proceedings. Currently, she is a post-doctoral researcher at the Agricultural University of Athens (AUA) and serves as an adjunct lecturer at the Department of Animal Sciences at the University of Thessaly for the lesson of “Biotechnology in animal husbandry and reproduction”. Her research interests focus on ruminant health and welfare, the laboratory diagnosis of infectious diseases in farm animals, and the quality and safety of produced milk and meat products.
Talk Title: Molecular methods for pathogen detection in farm animals: Application
The development of highly specific and robust diagnostic protocols for the molecular detection of pathogens in farm animals presents numerous challenges. During the presentation, the development of a highly sensitive and specific real-time PCR protocol for the detection of small ruminant lentiviruses in sheep will be analytically described as a characteristic example for the application of molecular methods for the detection of viral infections in farm animals. In particular, an holistic approach will be presented including the methods for the assessment of conventional PCR protocols, the sequencing and investigation of viral strains, the molecular characterization and phylogenetic analyses of viral strains, the use of bioinformatics tools, the designation and in silico evaluation of degenerate and specific primers for the circulating viral strains, the development and optimization of real-time PCR protocols, and the assessment of diagnostic performance of the newly developed real-time PCR protocols.
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This project is funded by the European Union under Horizon Europe GA 101159710